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Objective To explore the feasibility of clinical application of common day surgery in general surgery.Methods From January 2018 to December 2018,the clinical data of 40 cases of gallbladder stones (LC group),40 cases of breast lumps(breast lumps group) and 36 cases of mixed hemorrhoids(mixed hemorrhoids group) admitted to the daytime center of the First People's Hospital of Huainan were retrospectively analyzed.The average hospitalization days and expenses were analyzed,and each group was equally divided into control group (traditional surgery) and research group(day surgery).The hospitalization time and hospitalization expenses were compared between the control group and research group.Results The hospitalization time of the research group in the LC group,breast lumps group and mixed hemorrhoids group was (21.07 ± 6.31) h,(45.60 ± 7.39) h,(21.07 ±6.31) h,respectively,which were significantly shorter than those of the control group [(106.80 ± 25.20) h,(184.50 ± 25.00) h,(236.00 ± 54.14) h],the differences were statistically significant (t =15.093,14.898,-15.645,all P ＜ 0.05).The hospitalization expenses of the research group in the LC group,breast lumps group and mixed hemorrhoids group were (2 083.01 ± 436.04) CNY,(8 842.46 ± 861.15) CNY,(3 186.47 ± 464.93) CNY,respectively,which were also significantly lower than those of the control group [(3 594.82 ± 1 059.60) CNY,(11 003.76 ± 1 706.78) C NY,(6 419.69 ± 817.45) CNY],the differences were statistically significant (t =5.245,6.666,-16.082,all P ＜ 0.05).Conclusion Day surgery has obvious advantages and development space in clinical practice of common surgery in general surgery.It has incomparable effect and is superior to traditional surgery.
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Objective@#To explore the feasibility of clinical application of common day surgery in general surgery.@*Methods@#From January 2018 to December 2018, the clinical data of 40 cases of gallbladder stones(LC group), 40 cases of breast lumps(breast lumps group) and 36 cases of mixed hemorrhoids(mixed hemorrhoids group) admitted to the daytime center of the First People's Hospital of Huainan were retrospectively analyzed.The average hospitalization days and expenses were analyzed, and each group was equally divided into control group(traditional surgery) and research group(day surgery). The hospitalization time and hospitalization expenses were compared between the control group and research group.@*Results@#The hospitalization time of the research group in the LC group, breast lumps group and mixed hemorrhoids group was (21.07±6.31)h, (45.60±7.39)h, (21.07±6.31)h, respectively, which were significantly shorter than those of the control group[(106.80±25.20)h, (184.50±25.00)h, (236.00±54.14)h], the differences were statistically significant(t=15.093, 14.898, -15.645, all P<0.05). The hospitalization expenses of the research group in the LC group, breast lumps group and mixed hemorrhoids group were (2 083.01±436.04)CNY, (8 842.46±861.15)CNY, (3 186.47±464.93)CNY, respectively, which were also significantly lower than those of the control group[(3 594.82±1 059.60)CNY, (11 003.76±1 706.78)CNY, (6 419.69±817.45)CNY], the differences were statistically significant(t=5.245, 6.666, -16.082, all P<0.05).@*Conclusion@#Day surgery has obvious advantages and development space in clinical practice of common surgery in general surgery.It has incomparable effect and is superior to traditional surgery.
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Objective To assess the effect of neutrophil?to?lymphocyte ratio ( NLR) on the recurrence rate of patients with alpha?fetoprotein (AFP) ?negative hepatocellular carcinoma (HCC) and the value of NLR in predicting prognosis. Methods The clinical data of seventy?seven patients diagnosed with AFP?negative HCC and treated with hepatocellular carcinoma surgery in the First Hospital in Weinan from June 2015 to March 2017 were analyzed. According to the recurrence at the end of the follow?up, the patients were divided into the recurrence group and the non?recurrence group. Cox single factor analysis was used to analyze the relationship between the clinicopathological features and postoperative recurrence, and the clinical risk factors with statistically significance in the univariate analysis were placed in the Cox multivariate regression analysis to determine whether it is independent risk factor. Results The differences between the two groups in the number of tumors (31/15,28/3),tumor size (>5 cm) (5. 53±1. 83,4. 65±1. 73),portal vein tumor thrombus (18/28,2/29),microvascular invasion (14/32,2/29) were all statistically significant (P5 cm ) ( RR=0. 550, 95%CI 0. 316-0. 956, P=0. 038),portal vein tumor thrombus (RR=1. 294,95%CI 1. 208-1. 386,P=0. 022),microvascular invasion (RR=1. 575,95%CI1. 209-2. 052,P=0. 028) were the risk factors of postoperative recurrence. Cox regression model showed that tumor number (RR=1. 830,95%CI 1. 184-2. 828,P=0. 026),portal vein tumor thrombus ( RR=2. 860,95%CI 2. 062-3. 968,P=0. 001) ,microvascular invasion ( RR=1. 760,95%CI 1. 019-3. 041,P=0. 037) and preoperative NLR level ( RR=1. 890,95%CI 1. 056-3. 383,P=0. 028) were independent risk factors of the recurrence in AFP negative HCC patients after surgery. Among the 77 patients,46 cases were in the recurrent group, the average value of NLR was 3. 49 ± 0. 30, and the average preoperative NLR of the non?recurrence group ( 31 patients ) were 3. 01 ± 0. 30, the difference between the two groups in NLR value was statistically significant (t=-6. 885,P=0. 000). According to the ROC curve,the NLR=3. 17 corresponded to the maximum Youden index,the sensitivity of NLR was 82. 6%,the specificity was 67. 7%. Conclusion The preoperative NLR level is inversely proportional to the recurrence?free survival time of patients with AFP?negative HCC,which is one of the independent risk factors for recurrence. The optimal critical value of NLR is 3. 17.
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Objective To construct and identify the recombinant vector pcDNA3. 1 (-) B/myc-BRMS 1 carrying breast-cancer metastasis suppressor 1 (BRMS 1) which can express in eukaryote cells and which will provide the basis for further researching the mechanisms of metastasis suppression and working on cancer metastasis gene ther-apy. Methods To isolate total RNA from MCF - 7 cells and design a pair of primers, and coding sequence of aRMS 1 cDNA were amplified from human breast cancer cells MCF -7 by reverse transcription-polymerase chain reaction (RT-PCR). Then the product was inserted to the PcDNA3. 1/myc-His (-) B plasmid. The recombined pcDNA3. 1 (-)B/myc-BRMS1 was identified by gene sequence analysis,then recombinants was transfected into HEK-293 cells and was identified by Western blot. Results The recombinant of pcDNA3.1 (-) B/myc-BRMS1 was structurally confirmed by analysis of sequencing. The inserted fragment in the vector was in the right direction and its sequence was structurally confirmed to be consistent with CDS sequence of human BRMSI cDNA that of the published data. GenBank, [AF159141]. The recombinants was transfected into HEK-293 cells ,then the cells expressed protein tagged c-myc identified by Western blot indicated it can express in eukaryote cells. Conclusion cDNA of human BRMS1 can be successfully cloned and inserted into Eukaryote-expression vector. The newly constructed vector may serve as the potential tool to conduct further comprehensive experiments in future on BRMS1 function and on gene therapy.
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Objective To discuss the method of preventing injury of recurrent laryngeal nerve in thyroid surgery. Methods 362 cases of thyroid operation in our hospital were retrospectively studied, which consists of 188 cases with single lateral partial or total thyroidectomy, 127 with double lateral partial thyroidectomy,31 with single lateral total thyroidectomy plus isthmectomy, 16 with thyroid carcinoma radical correction. Results There was no injury,except for 3 cases damage to validate the recurrent laryngeal nerve in thyroid surgery,which were fully recov- ered after treatment. Conclusion To dissect recurrent laryngeal nerve routinely in inthyroid surgery can prevent and reduce the injury.